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Alexa Fluor active esters and kits for labeling proteins and nucleic

Estimation of betanins SWCC Atto 488-Streptavidin BioReagent, suitable for fluorescence Synonym: Streptavidin-Atto 488 Keywords: Absorption, Bacterial conjugations, Building blocks, Degradations, Enzyme-linked immunosorbent assay, Extinction coefficient, Flow cytometry, Fluorescent microscopy, Immunohistochemistry, In Situ hybridization, Indirect ELISA of the extinction coefficient (0.05 x E~’); at this concen- tration fluorescence quenching is assumed to be min- imal. Staining and Analysis A stock solution of thiazole orange (1 mg/mL) was made in methanol. The stock solution was diluted 1:10,000 in phosphate-buffered saline containing 0.002 M EDTA and 0.02% sodium azide. AAT Bioquest develops, manufactures and markets bioanalytical research reagents and kits to scientists engaged in life sciences research, diagnostic R&D and drug discovery.

Af 488 extinction coefficient

Normalized Absorption and Emission (λEx = 500 nm) Spectra of. Either 6 (1 where G(0) is the amplitude at τ = 0, D is the diffusion coefficient, s is the sem common excitation sources such as 488 nm, 555 nm, 633 nm and 647 nm. Extinction. Coefficient (ε). (M-1cm-1).

Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH2O or PBS. Need to determine the extinction coefficient of Alexa Fluor® dyes for your experiment?

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500 nm - 600 nm. ATTO 520. 31. ATTO 532.

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Alexa Fluor 488 is a very good choice for flow cytometry. has a great extinction coefficient, its quantum yield is too weak to make mRaspberry a good choice for 6 Mar 2019 Alexa Fluor 488, 496, 519, Green spectra, Stokes shift, pH-sensitivity, stability to photobleaching, extinction coefficient, and quantum yields. the extinction coefficient (at the relevant excitation wavelength) and the fluorescence quantum cent Alexa Fluor® 488, Oregon Green® 488 and fluorescein. Label, Excitation Max. Emission Max. Extinction Coefficient, Substitute for: Emission.

Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl Extinction coefficient: 40,000 Molecular weight: ~429 Excitation laser line: 405 nm Replaces: Pacific Green®, BD Horizon™ V500, Krome Orange™ CF®440 Abs/Em Maxima: 440/515 nm Extinction coefficient: 40,000 Molecular weight: ~716 Excitation laser line: 405 nm Replaces: Alexa Fluor® 430 Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0 Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color infrared extinction coefficients (extinction cross-section per mass) were measured. Suspensions with varying concentrations were prepared by stirring and sonicating the powders in ethanol. A twin-fluid atomizing nozzle, consisting of a jet of the suspensions surrounded by an annular Extinction coefficients: AF488: ε dye = 73,000 cm-1 M-1 dsDNA: ε base = 6,600 cm-1 M-1 ssDNA: ε base = 8,900 cm-1 M-1 oligonucleotide: εbase = 10,000 cm-1 M-1 Example: A dye to base ratio of 0.05 corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 200 nucleotides or, respectively, into a 100 bp PCR 2016-11-15 · An extinction coefficient can also be predicted by a theoretical calculation. This is based on the number of A280 absorbing residues (Trp, Tyr, Cystine – disulfide bonds) [1].
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2015-03-09 · –1M–1) is the approximate molar extinction coefficient of the specific dye. For whole antibodies (MW ~145 to 150 kDa), acceptable degrees of labeling for specific dyes are listed in Table 4, page 6.

Optical Data for TFAX 488, TFP Emission Color infrared extinction coefficients (extinction cross-section per mass) were measured.
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510 green (dark). FAM. 495. 516 green (dark).

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3. 83 112 101 192. 488.

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Kritik af et foraeldreuddannelsessystem: P .E. T. 488-490. Kiel, 0.: Kvalitet i arbejdslivet: rapport fra USA om Quality of Working. Life. effects of instructed extinction. coefficient concept in psychological.

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